Phytofabrication of bimetallic silver-copper/biochar nanocomposite for environmental and medical applications

Abstract

In the current study, a novel, green, low-cost, and sustainable path for the phyto-fabrication of Ag-Cu biochar nanocomposite (Ag-Cu/biochar) by Atriplex halimus biomass and aqueous extract is described. Surface plasmon resonance peaks were detected at 450 nm and 580 nm signifying the formation of both silver and copper nanoparticles, respectively on the biochar surface. XRD analysis confirmed the crystal structure of the phytosynthesized Ag-Cu/biochar whereas FT-IR, SEM, EDX, and XPS analyses confirmed the successful phytofabrication of the composite. Ag and Cu nanoparticles loaded on the biochar surface were almost spherically-shaped with a particle size ranging from 25 nm to 45 nm. Zeta potential of -25.5 mV showed the stability of Ag-Cu/biochar. The potential of this novel nanocomposite in the removal of doxycycline (DOX) was evident under different conditions as it reached nearly 100% under the optimum reaction conditions (DOX concentration; 50 ppm, pH; 9, a dose of Ag-Cu/biochar; 0.01 g, temperature; 25 °C, and H₂O₂ concentration; 100 mM). The promising regeneration of Ag-Cu/biochar was evident as the removal efficiency was 81% after 6 consecutive cycles. Ag-Cu/biochar was also shown an excellent antimicrobial activity against gram-negative bacteria as well a promising antioxidant activity.

Keywords: Antibacterial; Antioxidant; Biochar; Doxycycline; Green synthesis.