Transfection and transformation of Agrobacterium tumefaciens

Mol Gen Genet. 1978 Jul 11;163(2):181-7. doi: 10.1007/BF00267408.

Abstract

The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells.

MeSH terms

  • Bacteriological Techniques
  • Bacteriophages / genetics*
  • Coliphages / genetics
  • Microscopy, Electron
  • Rhizobium / genetics*
  • Rhizobium / ultrastructure
  • Transfection*
  • Transformation, Bacterial*