RNA-Seq analysis of duck embryo fibroblast cells gene expression during duck Tembusu virus infection

Vet Res. 2022 May 18;53(1):34. doi: 10.1186/s13567-022-01051-y.


Duck Tembusu virus (DTMUV), a member of the family Flaviviridae and an economically important pathogen with a broad host range, leads to markedly decreased egg production. However, the molecular mechanism underlying the host-DTMUV interaction remains unclear. Here, we performed high-throughput RNA sequencing (RNA-Seq) to study the dynamic changes in host gene expression at 12, 24, 36, 48 and 60 h post-infection (hpi) in duck embryo fibroblasts (DEF) infected with DTMUV. A total of 3129 differentially expressed genes (DEG) were identified after DTMUV infection. Gene Ontology (GO) category and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis revealed that these DEG were associated with multiple biological functions, including signal transduction, host immunity, virus infection, cell apoptosis, cell proliferation, and pathogenicity-related and metabolic process signaling pathways. This study analyzed viral infection and host immunity induced by DTMUV infection from a novel perspective, and the results provide valuable information regarding the mechanisms underlying host-DTMUV interactions, which will prove useful for the future development of antiviral drugs or vaccines for poultry, thus benefiting the entire poultry industry.

Keywords: RNA-seq; duck Tembusu virus; duck embryo fibroblast cells; immune responses; virus infection.

MeSH terms

  • Animals
  • Ducks
  • Fibroblasts
  • Flavivirus Infections* / veterinary
  • Flavivirus* / physiology
  • Gene Expression
  • Poultry Diseases*
  • Sequence Analysis, RNA / veterinary

Supplementary concepts

  • Tembusu virus