Separation of amino acids and antibiotics by narrow-bore and normal-bore high-performance liquid chromatography with pre-column derivatization

J Chromatogr. 1987 Jan 16;386:229-41. doi: 10.1016/s0021-9673(01)94600-x.


The selectivity, efficiency and lifetime of normal- and narrow-bore columns for high-performance liquid chromatography were investigated for the separation and quantification of amino acids and the amino acid-like antibiotics phosphinothricin and phosphinothricylalanylalanine in biological samples. These compounds were determined by an automated pre-column derivatization with o-phthalaldehyde-2-mercaptoethanol reagent and UV detection at 338 nm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / isolation & purification*
  • Anti-Bacterial Agents / isolation & purification*
  • Chemical Phenomena
  • Chemistry
  • Chromatography, High Pressure Liquid
  • Mercaptoethanol
  • Spectrophotometry, Ultraviolet
  • o-Phthalaldehyde


  • Amino Acids
  • Anti-Bacterial Agents
  • Mercaptoethanol
  • o-Phthalaldehyde