Single Cell Raman Spectroscopy Deuterium Isotope Probing for Rapid Antimicrobial Susceptibility Test of Elizabethkingia spp

Shuying Yuan; Yanwen Chen; Kaicheng Lin; Lin Zou; Xinrong Lu; Na He; Ruijie Liu; Shaoxing Zhang; Danfeng Shen; Zhenju Song; Chaoyang Tong; Yizhi Song; Wenhong Zhang; Li Chen; Guiqin Sun

doi:10.3389/fmicb.2022.876925

Single Cell Raman Spectroscopy Deuterium Isotope Probing for Rapid Antimicrobial Susceptibility Test of Elizabethkingia spp

Front Microbiol. 2022 May 3:13:876925. doi: 10.3389/fmicb.2022.876925. eCollection 2022.

Authors

Shuying Yuan¹, Yanwen Chen¹, Kaicheng Lin², Lin Zou³, Xinrong Lu³, Na He², Ruijie Liu¹, Shaoxing Zhang¹, Danfeng Shen³, Zhenju Song⁴, Chaoyang Tong⁴, Yizhi Song², Wenhong Zhang⁵, Li Chen³, Guiqin Sun¹

Affiliations

¹ School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, China.
² CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China.
³ Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.
⁴ Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
⁵ Department of Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China.

Abstract

Nosocomial infection by multi-drug resistance Elizabethkingia spp. is an emerging concern with severe clinical consequences, particularly in immunocompromised individuals and infants. Efficient control of this infection requires quick and reliable methods to determine the appropriate drugs for treatment. In this study, a total of 31 Elizabethkingia spp., including two standard strains (ATCC 13253 and FMS-007) and 29 clinical isolates obtained from hospitals in China were subjected to single cell Raman spectroscopy analysis coupled with deuterium probing (single cell Raman-DIP). The results demonstrated that single cell Raman-DIP could determine antimicrobial susceptibility of Elizabethkingia spp. in 4 h, only one third of the time required by standard broth microdilution method. The method could be integrated into current clinical protocol for sepsis and halve the report time. The study also confirmed that minocycline and levofloxacin are the first-line antimicrobials for Elizabethkingia spp. infection.

Keywords: Elizabethkingia spp.; antimicrobial susceptibility test; carbon-deuterium ratio; minimum inhibitory concentration; single cell Raman spectroscopy deuterium isotope probing.