FLOURY ENDOSPERM20 encoding SHMT4 is required for rice endosperm development

doi:10.1111/pbi.13858

. 2022 Aug;20(8):1438-1440.

doi: 10.1111/pbi.13858. Epub 2022 Jun 21.

FLOURY ENDOSPERM20 encoding SHMT4 is required for rice endosperm development

Mengyuan Yan^#¹, Tian Pan^#², Yun Zhu^#¹, Xiaokang Jiang², Mingzhou Yu², Rongqi Wang², Feng Zhang¹, Sheng Luo¹, Xiuhao Bao², Yu Chen¹, Binglei Zhang^{1

2}, Ruonan Jing², Zhijun Cheng¹, Xin Zhang¹, Cailin Lei¹, Qibing Lin¹, Shanshan Zhu¹, Xiuping Guo¹, Yulong Ren¹, Jianmin Wan^{1

2}

Affiliations

¹ National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
² State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.

^# Contributed equally.

PMID: 35596204
PMCID: PMC9342610
DOI: 10.1111/pbi.13858

Free PMC article

FLOURY ENDOSPERM20 encoding SHMT4 is required for rice endosperm development

Mengyuan Yan et al. Plant Biotechnol J. 2022 Aug.

Free PMC article

. 2022 Aug;20(8):1438-1440.

doi: 10.1111/pbi.13858. Epub 2022 Jun 21.

Authors

Affiliations

¹ National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
² State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, China.

^# Contributed equally.

PMID: 35596204
PMCID: PMC9342610
DOI: 10.1111/pbi.13858

No abstract available

Keywords: SAMS2; SHMT; endosperm development; methylation; rice.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
SHMT4 is required for rice endosperm development. (a) Full view and transverse sections of WT and *flo20‐1* seeds. (b–d) 1000‐brown kernel weight and physicochemical characteristics (b), SEM (c) and light microscopy (d) of WT and *flo20‐1*. (e) Gene structure of *SHMT4* and mutation sites of *flo20* alleles. Mutated nucleotides are shown in red. Putative splicing sites are underlined. (f) Complementation tests of *flo20‐1* grains. (g) Expression patterns of SHMT4. (h) Subcellular localization of SHMT4‐GFP. (i) Total SHMT activities in WT, *flo20‐1* and *CRI‐SHMT3* mutants. (j) The activity analysis of SHMT4 *in vitro*. (k–l) Y2H (k) and BiFC assay in tobacco (l) showing the interactions of SHMT4 and its homologues. (m) Summary of proteins co‐precipitated with SHMT4‐GFP, as identified by mass spectrometry. (n) The SHMT activity assay in SHMT4‐GFP and GFP transgenic rice. (o) Enzymatic activity of SHMT3&5 *in vitro*. (p) SHMT activity of SHMT4‐GST precipitate prepared from different background rice. (q) Verification of SHMT4 interacting with SAMS2 using BiFC and Co‐IP assays. (r) Measurement of SAM concentration in WT and *flo20‐1*. (s) Global distribution of DNA methylation levels over promoter, exon, intron and UTR of genes as well as repeat region. (t) RT‐qPCR analysis of *RPBF*, *RISBZ3*, *ONAC025*, *RISBZ1*, *ONAC020* and *ONAC026* in WT and *flo20‐1* endosperm at 10 DAF. Values given are means ± SD. Columns with different letters indicate significant differences (P < 0.05). Throughout, *P < 0.05, **P < 0.01, ***P < 0.001 by Student's t‐test. [Colour figure can be viewed at wileyonlinelibrary.com]

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References

1. Anderson, D.D. , Woeller, C.F. , Chiang, E.P. , Shane, B. and Stover, P.J. (2012) Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J. Biol. Chem. 287, 7051–7062. - PMC - PubMed
1. Ashida, K. , Saito, Y. , Masumura, T. and Iida, S. (2011) Ultrastructure of protein bodies in mutant rice (Oryza sativa L.) with altered storage protein composition. Breeding Sci. 61, 201–207.
1. Chen, J. , Huang, X.Y. , Salt, D.E. and Zhao, F.J. (2020) Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain. New Phytol. 226, 838–850. - PubMed
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1. Huang, L.C. , Tan, H.Y. , Zhang, C.Q. , Li, Q.F. et al. (2021) Starch biosynthesis in cereal endosperms: an updated review over the last decade. Plant Commun. 2, 100237. - PMC - PubMed

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[1] Anderson, D.D. , Woeller, C.F. , Chiang, E.P. , Shane, B. and Stover, P.J. (2012) Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J. Biol. Chem. 287, 7051–7062. - PMC - PubMed

[2] Anderson, D.D. , Woeller, C.F. , Chiang, E.P. , Shane, B. and Stover, P.J. (2012) Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J. Biol. Chem. 287, 7051–7062. - PMC - PubMed

[3] Ashida, K. , Saito, Y. , Masumura, T. and Iida, S. (2011) Ultrastructure of protein bodies in mutant rice (Oryza sativa L.) with altered storage protein composition. Breeding Sci. 61, 201–207.

[4] Ashida, K. , Saito, Y. , Masumura, T. and Iida, S. (2011) Ultrastructure of protein bodies in mutant rice (Oryza sativa L.) with altered storage protein composition. Breeding Sci. 61, 201–207.

[5] Chen, J. , Huang, X.Y. , Salt, D.E. and Zhao, F.J. (2020) Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain. New Phytol. 226, 838–850. - PubMed

[6] Chen, J. , Huang, X.Y. , Salt, D.E. and Zhao, F.J. (2020) Mutation in OsCADT1 enhances cadmium tolerance and enriches selenium in rice grain. New Phytol. 226, 838–850. - PubMed

[7] He, W. , Wang, L. , Lin, Q.L. and Yu, F. (2021) Rice seed storage proteins: biosynthetic pathways and the effects of environmental factors. J. Integr. Plant Biol. 63, 1999–2019. - PubMed

[8] He, W. , Wang, L. , Lin, Q.L. and Yu, F. (2021) Rice seed storage proteins: biosynthetic pathways and the effects of environmental factors. J. Integr. Plant Biol. 63, 1999–2019. - PubMed

[9] Huang, L.C. , Tan, H.Y. , Zhang, C.Q. , Li, Q.F. et al. (2021) Starch biosynthesis in cereal endosperms: an updated review over the last decade. Plant Commun. 2, 100237. - PMC - PubMed

[10] Huang, L.C. , Tan, H.Y. , Zhang, C.Q. , Li, Q.F. et al. (2021) Starch biosynthesis in cereal endosperms: an updated review over the last decade. Plant Commun. 2, 100237. - PMC - PubMed

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FLOURY ENDOSPERM20 encoding SHMT4 is required for rice endosperm development

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FLOURY ENDOSPERM20 encoding SHMT4 is required for rice endosperm development

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