Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

Aude Angelini; Xinchun Pi; Liang Xie

doi:10.1016/j.xpro.2022.101392

Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

STAR Protoc. 2022 May 17;3(2):101392. doi: 10.1016/j.xpro.2022.101392. eCollection 2022 Jun 17.

Authors

Aude Angelini^{1

2}, Xinchun Pi³, Liang Xie³

Affiliations

¹ Department of Medicine, Sections of Cardiovascular Research and Geriatrics, Baylor College of Medicine, Houston, TX 77030, USA.
² Cardiovascular Research Institute, Baylor College of Medicine, Houston, TX 77030, USA.
³ Department of Medicine, Section of Athero & Lipo, Baylor College of Medicine, Houston, TX 77030, USA.

Abstract

Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization. For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).

Keywords: Cell Biology; Cell culture; Cell isolation; Metabolism; Protein Biochemistry.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Animals, Newborn
Fatty Acids
Mice
Myocytes, Cardiac*
Respiration
Smegmamorpha*

Substances

Fatty Acids

Abstract

Publication types

MeSH terms

Substances

Grants and funding