Evaluation of long-chain fatty acid respiration in neonatal mouse cardiomyocytes using SeaHorse instrument

STAR Protoc. 2022 May 17;3(2):101392. doi: 10.1016/j.xpro.2022.101392. eCollection 2022 Jun 17.


Metabolic switches play a critical role in the pathophysiology of cardiac diseases, including heart failure. Here, we describe an assay for long-chain fatty acid oxidation in neonatal mouse cardiomyocytes by using a SeaHorse Flux Analyzer (Agilent). This protocol is a simplified but robust adaptation of the standard protocol that enables metabolic measurements in cells isolated from transgenic mouse models, which can be timesaving and informative. Cell isolation and culture represent a critical point that may require bench optimization. For complete details on the use and execution of this protocol, please refer to Angelini et al. (2021).

Keywords: Cell Biology; Cell culture; Cell isolation; Metabolism; Protein Biochemistry.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Animals, Newborn
  • Fatty Acids
  • Mice
  • Myocytes, Cardiac*
  • Respiration
  • Smegmamorpha*


  • Fatty Acids