The production of broad-spectrum antibiotics from phytopathogen Epicoccum sorghinum by culturing in host edible mushroom Thelephora ganbajun extract

Cui-Fang Wang; Xue-Qiong Yang; Wen-Zhi Shi; Xing Long; Shuang Su; Rong-Huan Cen; Ya-Bin Yang; Zhong-Tao Ding

doi:10.1016/j.phytochem.2022.113221

The production of broad-spectrum antibiotics from phytopathogen Epicoccum sorghinum by culturing in host edible mushroom Thelephora ganbajun extract

Phytochemistry. 2022 Aug:200:113221. doi: 10.1016/j.phytochem.2022.113221. Epub 2022 May 21.

Authors

Cui-Fang Wang¹, Xue-Qiong Yang¹, Wen-Zhi Shi¹, Xing Long¹, Shuang Su¹, Rong-Huan Cen¹, Ya-Bin Yang², Zhong-Tao Ding³

Affiliations

¹ Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China.
² Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China. Electronic address: ybyang@ynu.edu.cn.
³ Functional Molecules Analysis and Biotransformation Key Laboratory of Universities in Yunnan Province, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, PR China; College of Pharmacy, Dali University, Dali, 671003, PR China. Electronic address: ztding@ynu.edu.cn.

PMID: 35605808
DOI: 10.1016/j.phytochem.2022.113221

Abstract

Metabolism of special endophytes and phytopathogens can be induced by the symbiotic interactions with the host. A phytopathogen Epicoccum sorghinum cultured in host mushroom Thelephora ganbajun medium exhibited different metabolites compared with that of ordinary medium. An unprecedented scaffold possessing the same substructure as perylenequinone mycotoxin, a first methyl rearrangement product of phytotoxin, epoxydon 6-methylsalicylate ester, three undescribed compounds, and an undescribed natural product were isolated from E. sorghinum cultured in T. ganbajun. Episorin A and epicosorin A were produced from E. sorghinum induced by culturing in host medium. Episorin A was the first example of perylenequinone analogue in the natural products. These induced compounds and other metabolites showed notable antibiosis against endogenous fungi, and insect existing in mushroom. Induced episorin A showed significant inhibitory effects on nitric oxide production in LPS-activated macrophages, and anti-acetylcholinesterase with the IC₅₀ at 5.40 ± 0.25 μM, and 4.32 μM, respectively, and cytotoxicity against HL-60, A-549, SMMC-7721, MCF-7 and SW480 with IC₅₀ at 14.21 ± 0.53, 17.93 ± 0.22, 18.17 ± 0.63, 28.36 ± 0.43, and 18.20 ± 1.03 μM.

Keywords: Broad-spectrum antibiosis; Didymellaceae; Epicoccum sorghinum; Interaction; Metabolite; Thelephora ganbajun; Thelephoraceae.

MeSH terms

Agaricales* / chemistry
Anti-Bacterial Agents / metabolism
Ascomycota
Basidiomycota*

Substances

Anti-Bacterial Agents

Supplementary concepts

Epicoccum sorghinum
Thelephora ganbajun