Using ALLIGATORs to Capture Circadian Bioluminescence

Methods Mol Biol. 2022;2482:125-135. doi: 10.1007/978-1-0716-2249-0_8.

Abstract

Luciferases are a popular tool in circadian biology research as longitudinal reporters of gene expression. Here, we describe a short updated protocol for the use of an Automated Longitudinal Luciferase Imaging Gas and Temperature-Optimized Recorder (ALLIGATOR) to record cellular bioluminescence over many days. The ALLIGATOR has superior capacity and flexibility compared with traditional luminometers that employ photomultiplier tubes (PMTs), with high-throughput capability and spatial resolution. It can be readily adapted to a wide variety of applications, such as different sample types and plate sizes, under a wide range of physiologically relevant conditions.

Keywords: ALLIGATOR; Bioluminescence; Cellular clock biology; Circadian rhythm; Luciferase; PERIOD2.

MeSH terms

  • Alligators and Crocodiles*
  • Animals
  • Circadian Clocks*
  • Circadian Rhythm / physiology
  • Luciferases / genetics
  • Luciferases / metabolism
  • Luminescent Measurements / methods
  • Temperature

Substances

  • Luciferases