N-Terminomics/TAILS of Tissue and Liquid Biopsies

Methods Mol Biol. 2022:2456:85-94. doi: 10.1007/978-1-0716-2124-0_7.


The N-terminomics approach of Terminal Amine Isotopic Labeling of Substrates (TAILS) enables the identification and quantification of natural and neo-N-termini of proteins using liquid chromatography and tandem mass spectrometry (LC-MS/MS). This methodology has been used to study protease function and identify protease substrates in cell culture systems, animal disease models, and more recently, has been applied to clinical samples. Here, we present the application of TAILS to tissue and liquid biopsies.

Keywords: Biopsy; Mass spectrometry; N-terminomics; Protease; Proteomics; TAILS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines* / chemistry
  • Animals
  • Chromatography, Liquid
  • Isotope Labeling / methods
  • Liquid Biopsy
  • Peptide Hydrolases / metabolism
  • Proteome / metabolism
  • Proteomics* / methods
  • Tandem Mass Spectrometry / methods


  • Amines
  • Proteome
  • Peptide Hydrolases