Phosphoproteomics and Organelle Proteomics in Pancreatic Islets

Methods Mol Biol. 2022;2456:123-140. doi: 10.1007/978-1-0716-2124-0_9.

Abstract

Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.

Keywords: Mass spectrometry-based phosphoproteomics; Organelles; Pancreatic islets; Protein correlation profiling.

MeSH terms

  • Islets of Langerhans* / metabolism
  • Mass Spectrometry / methods
  • Organelles / metabolism
  • Phosphorylation
  • Proteomics* / methods