A Bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in Planta

Philippe V Jutras; Isobel Dodds; Renier A L van der Hoorn

doi:10.1007/978-1-0716-2241-4_15

A Bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in Planta

Methods Mol Biol. 2022;2480:285-293. doi: 10.1007/978-1-0716-2241-4_15.

Authors

Philippe V Jutras¹, Isobel Dodds¹, Renier A L van der Hoorn²

Affiliations

¹ Plant Chemetics Lab, Department of Plant Sciences, University of Oxford, Oxford, UK.
² Plant Chemetics Lab, Department of Plant Sciences, University of Oxford, Oxford, UK. renier.vanderhoorn@plants.ox.ac.uk.

PMID: 35616868
DOI: 10.1007/978-1-0716-2241-4_15

Abstract

Bioluminescence enables the monitoring of spatiotemporal dynamics and activity of bacterial populations in planta. We here describe a procedure to use AgroLux, a bioluminescent Agrobacterium tumefaciens, as a tool to study bacterial responses upon agroinfiltration. The first method details how to transform bioluminescent AgroLux to carry binary plasmids of interests. Then, a simple agroinfiltration assay for in planta imaging of bioluminescence signals is presented. AgroLux assays will increase our understanding of plant-Agrobacterium interactions and plant immunity and improve molecular farming.

Keywords: AgroLux; Agrobacterium tumefaciens; Agroinfiltration; Bacterial bioluminescence; Nicotiana benthamiana.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens* / metabolism
Plant Immunity*
Plasmids / genetics
Tobacco / genetics

Grants and funding

BB_/Biotechnology and Biological Sciences Research Council/United Kingdom