A Bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in Planta

Methods Mol Biol. 2022;2480:285-293. doi: 10.1007/978-1-0716-2241-4_15.


Bioluminescence enables the monitoring of spatiotemporal dynamics and activity of bacterial populations in planta. We here describe a procedure to use AgroLux, a bioluminescent Agrobacterium tumefaciens, as a tool to study bacterial responses upon agroinfiltration. The first method details how to transform bioluminescent AgroLux to carry binary plasmids of interests. Then, a simple agroinfiltration assay for in planta imaging of bioluminescence signals is presented. AgroLux assays will increase our understanding of plant-Agrobacterium interactions and plant immunity and improve molecular farming.

Keywords: AgroLux; Agrobacterium tumefaciens; Agroinfiltration; Bacterial bioluminescence; Nicotiana benthamiana.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens* / metabolism
  • Plant Immunity*
  • Plasmids / genetics
  • Tobacco / genetics