Diversified Polyketides With Anti-inflammatory Activities From Mangrove Endophytic Fungus Daldinia eschscholtzii KBJYZ-1

Guisheng Wang; Zhenhua Yin; Senye Wang; Yilin Yuan; Yan Chen; Wenyi Kang

doi:10.3389/fmicb.2022.900227

Diversified Polyketides With Anti-inflammatory Activities From Mangrove Endophytic Fungus Daldinia eschscholtzii KBJYZ-1

Front Microbiol. 2022 May 10:13:900227. doi: 10.3389/fmicb.2022.900227. eCollection 2022.

Authors

Guisheng Wang¹, Zhenhua Yin¹, Senye Wang¹, Yilin Yuan¹, Yan Chen^{1

2}, Wenyi Kang^{1

2

3}

Affiliations

¹ National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, China.
² Joint International Research Laboratory of Food and Medicine Resource Function, Kaifeng, China.
³ Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, China.

Abstract

In total, five new polyketide derivatives: eschscholin B (2), dalditone A and B (3 and 4), (1R, 4R)-5-methoxy-1,2,3,4-tetrahydronaphthalene-1,4-dio (5), and daldilene A (6), together with 10 known as analogs (1, 7-15) were isolated from the mangrove endophytic fungus Daldinia eschscholtzii KBJYZ-1. Their structures and absolute configurations were established by extensive analysis of NMR and HRESIMS spectra data combined with ECD calculations and the reported literature. Compounds 2 and 6 showed significant cell-based anti-inflammatory activities with IC₅₀ values of 19.3 and 12.9 μM, respectively. In addition, western blot results suggested that compound 2 effectively inhibits the expression of iNOS and COX-2 in LPS-induced RAW264.7 cells. Further molecular biology work revealed the potential mechanism of 2 exerts anti-inflammatory function by inactivating the MAPK and NF-κB signaling pathways.

Keywords: Daldinia eschscholtzii; MAPK; NF-κB; anti-inflammatory activity; mangrove endophytic fungus.