Method for the Intraoperative Detection of IDH Mutation in Gliomas with Differential Mobility Spectrometry

Curr Oncol. 2022 May 4;29(5):3252-3258. doi: 10.3390/curroncol29050265.

Abstract

Isocitrate dehydrogenase (IDH) mutation status is an important factor for surgical decision-making: patients with IDH-mutated tumors are more likely to have a good long-term prognosis, and thus favor aggressive resection with more survival benefit to gain. Patients with IDH wild-type tumors have generally poorer prognosis and, therefore, conservative resection to avoid neurological deficit is favored. Current histopathological analysis with frozen sections is unable to identify IDH mutation status intraoperatively, and more advanced methods are therefore needed. We examined a novel method suitable for intraoperative IDH mutation identification that is based on the differential mobility spectrometry (DMS) analysis of the tumor. We prospectively obtained tumor samples from 22 patients, including 11 IDH-mutated and 11 IDH wild-type tumors. The tumors were cut in 88 smaller specimens that were analyzed with DMS. With a linear discriminant analysis (LDA) algorithm, the DMS was able to classify tumor samples with 86% classification accuracy, 86% sensitivity, and 85% specificity. Our results show that DMS is able to differentiate IDH-mutated and IDH wild-type tumors with good accuracy in a setting suitable for intraoperative use, which makes it a promising novel solution for neurosurgical practice.

Keywords: classification; differential mobility spectrometry; glioma; isocitrate dehydrogenase (IDH); neuro-oncology; neurosurgery.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain Neoplasms* / diagnosis
  • Brain Neoplasms* / genetics
  • Brain Neoplasms* / surgery
  • Glioma* / genetics
  • Glioma* / surgery
  • Humans
  • Isocitrate Dehydrogenase / genetics
  • Mutation
  • Spectrum Analysis

Substances

  • Isocitrate Dehydrogenase

Grants and funding

Ilkka Haapala declares funding from The Finnish Medical Foundation, Eka -grant, decision number 3535. Anton Kondratev declares funding from Academy of Finland, Programmable Scent Environments -project, decision number 323530. Anton Kontunen declares funding from the Doctoral School of Tampere University and Emil Aaltonen Foundation (Grant number 210073). Markus Karjalainen declares funding from The Finnish Cultural Foundation, Pirkanmaa Regional Fund. Kristiina Nordfors declares funding from Finnish Pediatric Research Foundation, Pediatric Cancer Research Foundation Väre and The Finnish Ministry of Social Affairs and Health. Hannu Haapasalo declares funding from Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital. Niku Oksala declares funding from Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital (Grant numbers 9s045, 9T044, 9U042, 150618, 9V044, 9X040, 9AA057, 9AB052, and MK301); from Competitive funding to strengthen university research profiles funded by Academy of Finland, decision number 292477; and from Tampere Tuberculosis Foundation. Joonas Haapasalo declares funding from Emil Aaltonen Foundation, Finnish Pediatric Research Foundation, Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital, Pediatric Cancer Research Foundation Väre and The Finnish Ministry of Social Affairs and Health. The study sponsors did not have any involvement in the study design; collection, analysis, and interpretation of data; the writing of the manuscript; or the decision to submit the manuscript for publication.