Differential regional perfusion of the human anterior cruciate ligament: quantitative magnetic resonance imaging assessment

Kenneth M Lin; Harmen D Vermeijden; Craig E Klinger; Lionel E Lazaro; Scott A Rodeo; Jonathan P Dyke; David L Helfet; Gregory S DiFelice

doi:10.1186/s40634-022-00486-8

Differential regional perfusion of the human anterior cruciate ligament: quantitative magnetic resonance imaging assessment

J Exp Orthop. 2022 May 30;9(1):50. doi: 10.1186/s40634-022-00486-8.

Authors

Kenneth M Lin¹, Harmen D Vermeijden¹, Craig E Klinger¹, Lionel E Lazaro², Scott A Rodeo¹, Jonathan P Dyke³, David L Helfet¹, Gregory S DiFelice⁴

Affiliations

¹ Department of Orthopaedic Surgery, Hospital for Special Surgery, 535 East 70th Street, New York, NY, 10021, USA.
² Miami Orthopedics and Sports Medicine Institute, Baptist Health South Florida, Miami, FL, USA.
³ Citigroup Biomedical Imaging Center and Weill Cornell Medicine, New York, NY, USA.
⁴ Department of Orthopaedic Surgery, Hospital for Special Surgery, 535 East 70th Street, New York, NY, 10021, USA. difeliceg@hss.edu.

Abstract

Purpose: Surgical reconstruction is the current standard for ACL rupture treatment in active individuals. Recently, there is renewed interest in primary repair of proximal ACL tears. Despite this, ACL biology and healing potential are currently not well understood. Vascularity is paramount in ACL healing; however, previous ACL vascularity studies have been limited to qualitative histological and dissection-based techniques. The study objective was to use contrast-enhanced quantitative-MRI to compare relative perfusion of proximal, middle, and distal thirds of the in situ ACL. We hypothesized perfusion would be greatest in the proximal third.

Methods: Fourteen cadaveric knees were studied (8 females, 6 males), age 25-61 years. Superficial femoral, anterior tibial, and posterior tibial arteries were cannulated; without intraarticular dissection. Contrast-enhanced quantitative-MRI was performed using a previously established protocol. ACL regions corresponding to proximal, middle, and distal thirds were identified on sagittal-oblique pre-contrast images. Signal enhancement (normalized to tibial plateau cartilage) was quantified to represent regional perfusion as a percentage of total ACL perfusion. Comparative statistics were computed using repeated measures ANOVA, and pairwise comparisons performed using the Bonferroni method.

Results: Relative perfusion to proximal, middle, and distal ACL zones were 56.0% ±17.4%, 28.2% ±14.6%, and 15.8% ±16.3%, respectively (p = 0.002). Relative perfusion to the proximal third was significantly greater than middle (p = 0.007) and distal (p = 0.001). No statistically relevant difference in relative perfusion was found to middle and distal thirds (p = 0.281). Post-hoc subgroup analysis demonstrated greater proximal perfusion in males (66.9% ± 17.3%) than females (47.8% ± 13.0%), p = 0.036.

Conclusion: Using quantitative-MRI, in situ adult ACL demonstrated greatest relative perfusion to the proximal third, nearly 2 times greater than the middle third and 3 times greater than the distal third. Knowledge of differential ACL vascular supply is important for understanding pathogenesis of ACL injury and the process of biological healing following various forms of surgical treatment.

Keywords: ACL primary repair; ACL reconstruction; ACL vascularity; Perfusion; Quantitative MRI; Remnant preservation.

Grants and funding

NA/Hospital for Special Surgery Orthopaedic Trauma Research Fund