An Overview of SARS-CoV-2 Molecular Diagnostics in Europe

Clin Lab Med. 2022 Jun;42(2):161-191. doi: 10.1016/j.cll.2022.02.005. Epub 2022 Mar 8.

Abstract

The COVID-19 pandemic has led to the rapid development of a plethora of molecular diagnostic assays with real-time polymerase chain reaction (RT-PCR) at the forefront. In this review, we will discuss the history and utility of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) molecular diagnostics and the associated current and future regulatory process in Europe. We will assess the performance characteristics of a range of the most common SARS-CoV-2 molecular tests currently used in Europe with a focus on as rapid molecular platforms, stand-alone RT-PCR kits, the role of low-throughput and high-throughput end-to-end testing platforms, and the rapidly evolving field of SARS-CoV-2 variant of concern identification.

Keywords: CE Marking; COVID-19; Molecular diagnostics; RT-PCR; SARS-CoV-2.

Publication types

  • Review

MeSH terms

  • COVID-19* / diagnosis
  • Europe / epidemiology
  • Humans
  • Pandemics
  • Pathology, Molecular
  • SARS-CoV-2* / genetics

Supplementary concepts

  • SARS-CoV-2 variants