Novel Assays for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Kyle G Rodino; Kenneth P Smith; Matthew A Pettengill

doi:10.1016/j.cll.2022.02.004

Novel Assays for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2

Clin Lab Med. 2022 Jun;42(2):299-307. doi: 10.1016/j.cll.2022.02.004. Epub 2022 Mar 9.

Authors

Kyle G Rodino¹, Kenneth P Smith², Matthew A Pettengill³

Affiliations

¹ Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 3400 Spruce Street, 4th Floor Gates Building, Philadelphia, PA 19104, USA; Clinical Microbiology Laboratory, Hospital of the University of Pennsylvania, Philadelphia, PA, USA. Electronic address: https://twitter.com/KGRodinoPhD.
² Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Infectious Disease Diagnostics Laboratory, Children's Hospital of Philadelphia, Main Building 5NW91, 3401 Civic Center Boulevard, Philadelphia, PA 19104, USA.
³ Department of Pathology, Anatomy, and Cell Biology, Thomas Jefferson University, 117 South 11th Street, Pavilion Building, Suite 207, Philadelphia, PA 19107-4998, USA. Electronic address: matthew.pettengill@jefferson.edu.

Abstract

From the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)/COVID-19 pandemic, there has been a major emphasis on molecular laboratory tests for the virus. Shortages in various testing supplies, the desire to increase testing capacity, and a push to make point-of-care or home-based testing available have fostered considerable innovation for SARS-CoV-2 molecular diagnostics, advancements likely to be applicable to other diagnostic uses. The authors attempt to cover some of the most compelling novel types of molecular assays or novel approaches in adapting established molecular methodologies for SARS-CoV-2 detection or characterization.

Keywords: COVID-19; CRISPR-Cas; Microfluidics; Molecular; Next-generation sequencing; SARS-CoV-2; Variant.

Publication types

Review

MeSH terms

COVID-19* / diagnosis
Humans
Pandemics
SARS-CoV-2*