NT-seq: a chemical-based sequencing method for genomic methylome profiling

Genome Biol. 2022 May 30;23(1):122. doi: 10.1186/s13059-022-02689-9.


DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N6-methyladenine (6mA), N4-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation.

Keywords: DNA methylation; Next-generation sequencing; Whole-genome epigenetic profiling.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • 5-Methylcytosine*
  • DNA Methylation
  • Epigenome*
  • Genome, Bacterial
  • Genomics


  • 5-Methylcytosine