The in vivo fate and targeting engineering of crossover vesicle-based gene delivery system

Xin-Chi Jiang; Tianyuan Zhang; Jian-Qing Gao

doi:10.1016/j.addr.2022.114324

The in vivo fate and targeting engineering of crossover vesicle-based gene delivery system

Adv Drug Deliv Rev. 2022 Aug:187:114324. doi: 10.1016/j.addr.2022.114324. Epub 2022 May 29.

Authors

Xin-Chi Jiang¹, Tianyuan Zhang¹, Jian-Qing Gao²

Affiliations

¹ College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
² College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Hangzhou Institute of Innovative Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, PR China; Department of Pharmacy, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, PR China. Electronic address: gaojianqing@zju.edu.cn.

PMID: 35640803
DOI: 10.1016/j.addr.2022.114324

Abstract

Exosomes and biomimetic vesicles are widely used for gene delivery because of their excellent gene loading capacity and stability and their natural targeting delivery potential. These vesicles take advantages of both cell-based bioactive delivery system and synthetical lipid-derived nanovectors to form crossover characteristics. To further optimize the specific targeting properties of crossover vesicles, studies of their in vivo fate and various engineering approaches including nanobiotechnology are required. This review describes the preparation process of exosomes and biomimetic vesicles, and summarizes the mechanism of loading and delivery of nucleic acids or gene editing systems. We provide a comprehensive overview of the techniques employed for preparing the targeting crossover vesicles based on their cellular uptake and targeting mechanism. To delineate the future prospects of crossover vesicle gene delivery systems, various challenges and clinical applications of vesicles have also been discussed.

Keywords: Biomimetic vesicles; CRISPR/Cas9; Exosomes; Gene delivery system; Targeting.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Drug Delivery Systems / methods
Exosomes*
Extracellular Vesicles*
Gene Editing
Gene Transfer Techniques
Genetic Therapy
Humans
Nucleic Acids*

Substances

Nucleic Acids