Gut bacterial isoamylamine promotes age-related cognitive dysfunction by promoting microglial cell death

Cell Host Microbe. 2022 Jul 13;30(7):944-960.e8. doi: 10.1016/j.chom.2022.05.005. Epub 2022 Jun 1.


The intestinal microbiome releases a plethora of small molecules. Here, we show that the Ruminococcaceae metabolite isoamylamine (IAA) is enriched in aged mice and elderly people, whereas Ruminococcaceae phages, belonging to the Myoviridae family, are reduced. Young mice orally administered IAA show cognitive decline, whereas Myoviridae phage administration reduces IAA levels. Mechanistically, IAA promotes apoptosis of microglial cells by recruiting the transcriptional regulator p53 to the S100A8 promoter region. Specifically, IAA recognizes and binds the S100A8 promoter region to facilitate the unwinding of its self-complementary hairpin structure, thereby subsequently enabling p53 to access the S100A8 promoter and enhance S100A8 expression. Thus, our findings provide evidence that small molecules released from the gut microbiome can directly bind genomic DNA and act as transcriptional coregulators by recruiting transcription factors. These findings further unveil a molecular mechanism that connects gut metabolism to gene expression in the brain with implications for disease development.

Keywords: DNA unwinding; aged brain; binding promoter; cognitive dysfunction; gut bacterial metabolite; gut bacteriophages; isoamylamine; microglial; sensome gene S100A8; transcription factor p53.

MeSH terms

  • Amines
  • Animals
  • Bacteria
  • Bacteriophages* / genetics
  • Cognitive Dysfunction*
  • Gastrointestinal Microbiome*
  • Humans
  • Mice
  • Microglia
  • Tumor Suppressor Protein p53


  • Amines
  • Tumor Suppressor Protein p53
  • isoamylamine