Release of core DNA from nucleosomal core particles following (ADP-ribose)n-modification in vitro

Biochem Biophys Res Commun. 1987 Mar 30;143(3):1049-54. doi: 10.1016/0006-291x(87)90358-5.

Abstract

Isolated nucleosomal core particles from rat liver chromatin were ADP-ribosylated in vitro and the consequences of this modification on intranucleosomal DNA-protein interactions were studied by retention gel analysis. A separating force of 11 fN caused the complete release of 146 bp DNA fragments from ADP-ribosylated core particles, while the DNA of unmodified core particles remained protein-associated. We conclude that posttranslational (ADP-ribose)n-modification of chromatin proteins may reduce DNA-protein interactions at the nucleosomal level of chromatin organization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate Ribose / metabolism*
  • Animals
  • Chromatin / metabolism
  • DNA / metabolism*
  • In Vitro Techniques
  • Liver / metabolism
  • Male
  • Nucleosomes / metabolism*
  • Protein Processing, Post-Translational
  • Rats

Substances

  • Chromatin
  • Nucleosomes
  • Adenosine Diphosphate Ribose
  • DNA