Abstract
All four subunits of the acetylcholine receptor in membrane vesicles isolated from Torpedo californica have been labeled with [3H]cholesteryl diazoacetate. As this probe incorporates into lipid bilayers analogously to cholesterol, this result indicates that acetylcholine receptor interacts with cholesterol. This investigation also demonstrates that this probe is a useful reagent for studying the interaction of cholesterol with membrane proteins.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Affinity Labels
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Animals
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Azo Compounds
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Cell Membrane / ultrastructure
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Cholesterol / metabolism*
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Cholesterol Esters
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Diazonium Compounds*
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Macromolecular Substances
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Membrane Lipids / metabolism
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Photolysis
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Protein Conformation
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Receptors, Nicotinic* / metabolism*
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Torpedo
Substances
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Affinity Labels
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Azo Compounds
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Cholesterol Esters
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Diazonium Compounds
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Macromolecular Substances
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Membrane Lipids
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Receptors, Nicotinic
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cholesteryl diazoacetate
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Cholesterol