Carbachol-induced EEG 'theta' activity in hippocampal brain slices

Brain Res. 1987 Mar 3;405(1):196-8. doi: 10.1016/0006-8993(87)91009-2.


Application of the cholinergic agonist carbachol (50 microM) produced theta-like rhythmical waveforms, recorded in the stratum moleculare of the dentate gyrus. Atropine sulfate (50 microM) antagonized the carbachol-induced theta-like activity, consistent with this action of atropine in vivo. These results provide the first direct evidence that hippocampal neurons are capable of producing synchronized slow-wave activity when isolated from pulsed rhythmic inputs of the medial septum and other brain regions.

MeSH terms

  • Animals
  • Atropine / pharmacology
  • Biological Clocks
  • Carbachol / pharmacology*
  • Cholinergic Fibers / physiology
  • Electroencephalography*
  • Hippocampus / drug effects*
  • In Vitro Techniques
  • Neural Pathways / physiology
  • Rats
  • Rats, Inbred Strains
  • Septum Pellucidum / physiology
  • Theta Rhythm*


  • Atropine
  • Carbachol