Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells

Chia-Hung Christine Hsiao; Andrew J Wiemer

doi:10.1016/j.xpro.2022.101422

Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells

STAR Protoc. 2022 Jun 4;3(2):101422. doi: 10.1016/j.xpro.2022.101422. eCollection 2022 Jun 17.

Authors

Chia-Hung Christine Hsiao¹, Andrew J Wiemer^{1

2}

Affiliations

¹ Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA.
² Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269, USA.

Abstract

Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat and describes how to assess their activation by antigen-containing target cells. While specifically focused on cytokine production, this protocol can be readily adapted to evaluate other effector functions of activated Vγ9Vδ2 T cells. For complete details on the use and execution of this protocol, please refer to Hsiao et al. (2022) and Hsiao and Wiemer (2018).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Cell separation/fractionation; Cell-based Assays; Immunology.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Antigens
Butyrophilins / genetics
Humans
Lymphocyte Activation*
T-Lymphocytes*

Substances

Antigens
Butyrophilins

Abstract

Publication types

MeSH terms

Substances

Grants and funding