Poly-l-lactic acid (PLLA)/anthocyanin nanofiber color indicator film for headspace detection of low-level bacterial concentration

Wenbo Li; Wuliang Sun; Lu Jia; Yue Dong; Lingling Wu; Marleny D A Saldaña; Wenxiu Sun

doi:10.1016/j.ijbiomac.2022.06.034

Poly-l-lactic acid (PLLA)/anthocyanin nanofiber color indicator film for headspace detection of low-level bacterial concentration

Int J Biol Macromol. 2022 Aug 31:215:123-131. doi: 10.1016/j.ijbiomac.2022.06.034. Epub 2022 Jun 9.

Authors

Wenbo Li¹, Wuliang Sun², Lu Jia¹, Yue Dong¹, Lingling Wu¹, Marleny D A Saldaña³, Wenxiu Sun⁴

Affiliations

¹ College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.
² College of Materials Science and Engineering, Inner Mongolia University of Technology, Hohhot 010051, China.
³ Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, T6G 2P5 Edmonton, AB, Canada.
⁴ College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; Department of Agricultural, Food and Nutritional Science (AFNS), University of Alberta, T6G 2P5 Edmonton, AB, Canada. Electronic address: wenxiu_sun@imau.edu.cn.

PMID: 35691434
DOI: 10.1016/j.ijbiomac.2022.06.034

Abstract

Since bacterial contamination is a significant threat to humans, early detection is essential to safeguard dietary safety and physical health. Here, a nanofiber color indicator film based on poly-l-lactic acid (PLLA) as the support and anthocyanin as the indicator material was prepared by electrostatic spinning. It was found that the PLLA/0.8CY nanofiber color indicator film was hydrophobic (the water contact angle of 102.4°) and contained uniform nanofibers with an average diameter of 750 nm. In addition, the film's humidity insensitivity, reusability, color stability, and ammonia sensitivity (the limits of detection 35.39 ppm) made the film environmentally friendly and more accurate and faster for bacterial detection. The film was able to sense 10² CFU/mL of gram-positive and negative bacteria after the model strain E. coli and L. monocytogene. Thus, the PLLA/0.8CY nanofiber color indicator film was able to perform headspace nondestructive detection of low-level bacterial contamination.

Keywords: Color indicator film; Electrostatic spinning; Low-level bacterial detection.

MeSH terms

Anthocyanins
Escherichia coli
Humans
Nanofibers* / chemistry
Polyesters / chemistry

Substances

Anthocyanins
poly(lactide)
Polyesters