Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 May 25:13:845038.
doi: 10.3389/fmicb.2022.845038. eCollection 2022.

Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals

Ikram Khan  1   2 , Imran Khan  3 , Mian Adnan Kakakhel  1 , Zhang Xiaowei  4 , Mao Ting  4 , Ikram Ali  1 , Yu Fei  2 , Zhou Jianye  2 , Li Zhiqiang  2 , An Lizhe  1
Affiliations

Comparison of Microbial Populations in the Blood of Patients With Myocardial Infarction and Healthy Individuals

Ikram Khan et al. Front Microbiol. .

Abstract

Increased bacterial translocation in the gut and bloodstream infections are both major comorbidities of heart failure and myocardial infarction (MI). However, the alterations in the microbiome of the blood of patients with MI remain unclear. To test this hypothesis, we conducted this case-control study to explore the microbiota compositions in the blood of Chinese patients with MI. Using high-throughput Illumina HiSeq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene, the microbiota communities in the blood of 29 patients with MI and 29 healthy controls were examined. In addition, the relationship between the blood microbiome and clinical features of MI was investigated. This study revealed a significant reduction in alpha diversity (Shannon index) in the MI group compared with the healthy controls. Also, a significant difference was detected in the structure and richness between the patients with MI and healthy controls. The members of the phylum Actinobacteria, class Actinobacteria, order Bifdobacteriales, family Bifidobacteriaceae, and genus Bifidobacterium were significantly abundant in the MI group, while the members of the phylum Bacteroidetes, class Bacteroidia, and order Bacteroidales were significantly enriched in the healthy controls (p < 0.05). Moreover, the functional analysis revealed a significant variation between both groups. For instance, the enrichment of genes involved in the metabolism pathways of three amino acids decreased, that is, nucleotide transport and metabolism, coenzyme transport and metabolism, and lipid transport and metabolism, among others. Our study will contribute to a better knowledge of the microbiota of blood, which will further lead to improved MI diagnosis and therapy. Further study is needed to determine the role of the blood microbiota in human health and disease.

Keywords: HiSeq; bacteria; blood circulation; functional analysis; myocardial infarction.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
The Venn diagram represents the shared and unique OTUs in both MI and HC groups.
Figure 2
Figure 2
Phylum proportion of HC and MI groups. Pie charts indicate the percentages of each of the top 10 high-abundance phyla in both groups.
Figure 3
Figure 3
The genus-level abundance of HC and MI groups. Pie charts indicate the percentages of each of the top 10 high-abundance genera in both groups.
Figure 4
Figure 4
(A) Principal coordinate analysis (PCoA) of the overall composition of the genera communities among both groups. (B) PERMANOVA analysis indicates variation in blood bacterial species between HC and MI groups.
Figure 5
Figure 5
(A) LEfSe analysis plot of differentially abundant blood microbial taxa between MI and control. (B) The taxonomic tree of differentially abundant taxa is represented by the cladogram.
Figure 6
Figure 6
The correlation between clinical parameters and blood bacterial taxa.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Ahmadmehrabi S., Tang W. H. W. (2017). Gut microbiome and its role in cardiovascular diseases. Curr. Opin. Cardiol. 32, 761–766. 10.1097/HCO.0000000000000445 - DOI - PMC - PubMed
    1. Amar J., Chabo C., Waget A., Klopp P., Vachoux C., Bermúdez-Humarán L. G., et al. . (2011a). Intestinal mucosal adherence and translocation of commensal bacteria at the early onset of type 2 diabetes: Molecular mechanisms and probiotic treatment. EMBO Mol. Med. 3, 559–572. 10.1002/emmm.201100159 - DOI - PMC - PubMed
    1. Amar J., Lange C., Payros G., Garret C., Chabo C., Lantieri O., et al. . (2013). Blood microbiota dysbiosis is associated with the onset of cardiovascular events in a large general population: the DESIR study. PLoS ONE 8:e54461. 10.1371/journal.pone.0054461 - DOI - PMC - PubMed
    1. Amar J., Lelouvier B., Servant F., Lluch J., Burcelin R., Bongard V., et al. . (2019). Blood microbiota modification after myocardial infarction depends upon low-density lipoprotein cholesterol levels. J. Am. Heart Assoc. 8:e011797. 10.1161/JAHA.118.011797 - DOI - PMC - PubMed
    1. Amar J., Serino M., Lange C., Chabo C., Iacovoni J., Mondot S., et al. . (2011b). Involvement of tissue bacteria in the onset of diabetes in humans: evidence for a concept. Diabetologia 54, 3055–3061. 10.1007/s00125-011-2329-8 - DOI - PubMed

LinkOut - more resources