High-level, heat-regulated synthesis of proteins in eukaryotic cells

Gene. 1986;49(1):1-8. doi: 10.1016/0378-1119(86)90380-x.


Plasmids have been constructed in which promoters of 70-kDa heat-shock protein genes (hsp70) of human and Drosophila origin were linked to three different eukaryotic genes encoding human growth hormone (hGH), chicken lysozyme (cL) and a human influenza haemagglutinin (HA). Following transfection into widely divergent eukaryotic cells, the hybrid genes direct the transient, heat-regulated synthesis of the three proteins. hGH and cL are secreted into the medium. A human hsp70-hGH construct was used to establish stable mouse fibroblast lines that are capable of producing and secreting hGH at high levels following heat induction: hGH is secreted at a 500-1200-fold higher rate by heat-treated than by untreated cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Cells, Cultured
  • Cloning, Molecular
  • Drosophila / genetics
  • Female
  • Genes*
  • Genetic Linkage*
  • Growth Hormone / genetics
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics*
  • Hemagglutinins, Viral / genetics
  • Hot Temperature
  • Humans
  • Mice
  • Muramidase / genetics
  • Nucleic Acid Hybridization
  • Oocytes / metabolism
  • Plasmids
  • Promoter Regions, Genetic
  • Xenopus


  • Heat-Shock Proteins
  • Hemagglutinins, Viral
  • Growth Hormone
  • Muramidase