A novel intra- and extracellular distribution pattern of elemental sulfur in Pseudomonas sp. C27-driven denitrifying sulfide removal process

Kaili Fan; Xijun Xu; Fan Xu; Jia Shi; Kai Sun; Irina Fedorova; Nanqi Ren; Duu-Jong Lee; Chuan Chen

doi:10.1016/j.envres.2022.113674

A novel intra- and extracellular distribution pattern of elemental sulfur in Pseudomonas sp. C27-driven denitrifying sulfide removal process

Environ Res. 2022 Oct:213:113674. doi: 10.1016/j.envres.2022.113674. Epub 2022 Jun 11.

Authors

Kaili Fan¹, Xijun Xu¹, Fan Xu¹, Jia Shi¹, Kai Sun¹, Irina Fedorova², Nanqi Ren¹, Duu-Jong Lee³, Chuan Chen⁴

Affiliations

¹ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150090, China.
² Department of Geoecology and Natural Resource Management, Saint Petersburg State University, Institute of Earth Science, Russia.
³ Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan.
⁴ State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150090, China. Electronic address: echo110244@126.com.

PMID: 35700768
DOI: 10.1016/j.envres.2022.113674

Abstract

Pseudomonas sp. C27 can achieve the conversion of toxic sulfide to economical elemental sulfur (S⁰) with various electron acceptors. In this study the distribution pattern of S⁰ produced by C27 in denitrifying sulfide removal (DSR) process was explored. The SEM observation identified that the particle size of the biogenic S⁰ was at micron level. Strikingly, a novel distribution pattern of S⁰ was revealed that the produced S⁰ was not directly secreted extracellularly, but be stored temporarily in the cell interior. Pyrolysis at 65 °C for 20 min were recommended prior to S⁰ recovery, which could maximize the separation of extracellular polymeric substances (EPS) from C27. Furthermore, the effects of N/S molar ratio, initial sulfide concentration, and micro-oxygen condition were investigated to improve the production of S⁰ by C27. The highest S⁰ production was obtained at S/N of 3 and anaerobic condition seemed to favor the S⁰ production by C27. This study would provide a theoretical support for highly efficient sulfide removal as well as S⁰ recovery in sulfide-laden wastewater treatment.

Keywords: Denitrifying sulfide removal; Elemental sulfur; Pseudomonas sp. C27; Sulfur oxidizing bacteria.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bioreactors
Denitrification
Nitrates
Pseudomonas*
Sulfides
Sulfur
Water Purification*

Substances

Nitrates
Sulfides
Sulfur