Methodological considerations for circulating long noncoding RNA quantification

David de Gonzalo-Calvo; Miron Sopić; Yvan Devaux; EU-CardioRNA COST Action CA17129

doi:10.1016/j.molmed.2022.05.011

Methodological considerations for circulating long noncoding RNA quantification

Trends Mol Med. 2022 Aug;28(8):616-618. doi: 10.1016/j.molmed.2022.05.011. Epub 2022 Jun 11.

Authors

David de Gonzalo-Calvo¹, Miron Sopić², Yvan Devaux³; EU-CardioRNA COST Action CA17129

Affiliations

¹ Translational Research in Respiratory Medicine, University Hospital Arnau de Vilanova and Santa Maria, IRBLleida, Lleida, Spain; CIBER of Respiratory Diseases (CIBERES), Institute of Health Carlos III, Madrid, Spain. Electronic address: dgonzalo@irblleida.cat.
² Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. Electronic address: miron.sopic@pharmacy.bg.ac.rs.
³ Cardiovascular Research Unit, Department of Population Health, Luxembourg Institute of Health, Strassen, Luxembourg.

PMID: 35701316
DOI: 10.1016/j.molmed.2022.05.011

Abstract

In the past decade, significant resources have been invested in long noncoding RNA (lncRNA) research. Despite the knowledge available, we are far from incorporation of lncRNA into clinical practice. Here, we emphasize the technical challenges in the field, hoping to provoke a response leading to new consensus and guidelines.

Keywords: RT-qPCR; biomarker; long noncoding RNA; methods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
RNA, Long Noncoding* / genetics
RNA, Long Noncoding* / metabolism

Substances

RNA, Long Noncoding