Inhibition of polyclonal B-cell activation by suppressor monocytes in patients with sarcoidosis

Clin Exp Immunol. 1978 Jun;32(3):554-62.

Abstract

The present study employed a direct plaque-forming cell (PGC) assay following pokeweed mitogen (PWM) induced polyclonal activation of B lymphocytes in sarcoidosis to evaluate the in vitro humoral immune response in this disease and to delineate the immunoregulation of this response. Sarcoidosis lymphocytes had a suppressed PFC response to polyclonal activation, but were unable to suppress normal B-cell PFC responses in allogeneic co-cultures. Removal of a cell type, which was corticosteroid-resistant, radio-resistant, adherent and a non-T cell, from sarcoidosis mononuclear cell suspensions reversed the suppressed PFC response, indicating the presence of a suppressor monocyte. Thus in vitro suppressor cell activity has now been demonstrated in this disease, which is characterized by multiple immunological aberrancies.

MeSH terms

  • Antibody Formation*
  • B-Lymphocytes / immunology*
  • Clone Cells
  • Hemolytic Plaque Technique
  • Humans
  • Hydrocortisone / pharmacology
  • Immunosuppression
  • Lymphocyte Activation
  • Lymphocytes / drug effects
  • Lymphocytes / radiation effects
  • Monocytes / immunology*
  • Pokeweed Mitogens / pharmacology
  • Sarcoidosis / immunology*
  • X-Rays

Substances

  • Pokeweed Mitogens
  • Hydrocortisone