BTDAzo: A Photoswitchable TRPC5 Channel Activator

Angew Chem Int Ed Engl. 2022 Sep 5;61(36):e202201565. doi: 10.1002/anie.202201565. Epub 2022 Jul 27.


Photoswitchable reagents can be powerful tools for high-precision biological control. TRPC5 is a Ca2+ -permeable cation channel with distinct tissue-specific roles, from synaptic function to hormone regulation. Reagents giving spatiotemporally-resolved control over TRPC5 activity may be key to understanding and harnessing its biology. Here we develop the first photoswitchable TRPC5-modulator, BTDAzo, to address this goal. BTDAzo can photocontrol TRPC5 currents in cell culture, as well as controlling endogenous TRPC5-based neuronal Ca2+ responses in mouse brain slices. BTDAzos are also the first reported azo-benzothiadiazines, an accessible and conveniently derivatised azoheteroarene with strong two-colour photoswitching. BTDAzo's ability to control TRPC5 across relevant channel biology settings makes it suitable for a range of dynamically reversible photoswitching studies in TRP channel biology, with the aim to decipher the various biological roles of this centrally important ion channel.

Keywords: Agonists; Cation Channels; Ligand Design; Photopharmacology; Prolactin Signaling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism
  • Mice
  • Neurons* / metabolism
  • TRPC Cation Channels*


  • TRPC Cation Channels
  • Trpc5 protein, mouse
  • Calcium