D-ribose-L-cysteine exhibits neuroprotective activity through inhibition of oxido-behavioral dysfunctions and modulated activities of neurotransmitters in the cerebellum of Juvenile mice exposed to ethanol

Drug Chem Toxicol. 2023 Nov;46(4):746-756. doi: 10.1080/01480545.2022.2088783. Epub 2022 Jun 20.


Alcohol exposure to the cerebellum has been known to trigger cerebellar dysfunctions through several mechanisms. This present study was designed to evaluate the repealing effect of D-ribose-L-cysteine (DRLC) on alcohol-induced cerebellar dysfunctions in juvenile BALB/c mice. The animals were randomly divided into 4 groups (n = 10 per group). Mice were given oral administration of normal saline (control), DRLC (100 mg/kg, p.o), ethanol (0.2 mL of 10% w/v), or DRLC (100 mg/kg, p.o) + ethanol (0.2 mL of 10% w/v). On day 29 of the study (i.e., 24 h after the administration of the last respective doses), neurochemical quantification of the respective levels of serotonin and dopamine, lipid peroxidation, total antioxidant, superoxide dismutase, and glutathione peroxidase in the cerebellar tissues of the mice were analyzed. Compared with the saline-treated group, the studied neurochemical indices were modulated across the various experimental groups. The administration of ethanol significantly modulates the levels of monoamine neurotransmitters (serotonin and dopamine) as well as contents of total antioxidants, activities of superoxide dismutase, and glutathione peroxidase, with a concurrently increased level of lipid peroxidase in the cerebellar tissue of the mice. DRLC significantly reverses these effects in the DRLC + ethanol co-treated group. Combined exposure to DRLC + ethanol counteracts the deleterious effect of ethanol in the cerebellum of juvenile BALB/c mice via monoamine neurotransmitter, lipid peroxidation, total antioxidant status, superoxide dismutase, and glutathione peroxidase action pathways. Therefore, DRLC could be a pharmacologic or therapeutic agent in attenuating the deleterious effects of alcohol on the cerebellum.

Keywords: Purkinje neurons; Ribose-cysteine; alcohol; dopamine; serotonin.

MeSH terms

  • Animals
  • Antioxidants* / metabolism
  • Antioxidants* / pharmacology
  • Catalase / metabolism
  • Cerebellar Diseases* / metabolism
  • Cerebellum / metabolism
  • Dopamine / metabolism
  • Ethanol / toxicity
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation
  • Mice
  • Oxidative Stress
  • Serotonin
  • Superoxide Dismutase / metabolism


  • 2-(1',2',3',4'-tetrahydroxybutyl)thiazolidine-4-carboxylic acid
  • Antioxidants
  • Catalase
  • Dopamine
  • Ethanol
  • Glutathione Peroxidase
  • Serotonin
  • Superoxide Dismutase