Isolation of UV-Resistant Marine Bacteria by UV-C Assays

Methods Mol Biol. 2022:2498:293-305. doi: 10.1007/978-1-0716-2313-8_15.

Abstract

Marine organisms have developed physiological and biochemical strategies to survive under the exposure of UV-B radiation. In particular, Antarctic marine bacteria, exposed to extremes of temperature, UV and ice, have adapted to cope with UV radiation by producing photoprotective molecules. Here, we describe (1) the sampling strategy to collect marine samples (surface water/ice and sediment samples) and (2) the selection strategy to isolate in these samples only UV-resistant marine bacteria.

Keywords: Bioactive compound; Isolation strategy; Marine bacterium; Photoprotection; UV radiation; UV-C assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aquatic Organisms
  • Bacteria / genetics
  • Ice*
  • Ultraviolet Rays*

Substances

  • Ice