Binding of the extracellular matrix laminin-1 to Clostridioides difficile strains

Mem Inst Oswaldo Cruz. 2022 Jun 17;117:e220035. doi: 10.1590/0074-02760220035. eCollection 2022.


Background: Clostridioides difficile is the most common cause of nosocomial diarrhea associated with antibiotic use. The disease's symptoms are caused by enterotoxins, but other surface adhesion factors also play a role in the pathogenesis. These adhesins will bind to components of extracellular matrix.

Objective: There is a lack of knowledge on MSCRAMM, this work set-out to determine the adhesive properties of several C. difficile ribotypes (027, 133, 135, 014, 012) towards laminin-1 (LMN-1).

Methods: A binding experiment revealed that different ribotypes have distinct adhesion capabilities. To identify this adhesin, an affinity chromatography column containing LMN-1 was prepared and total protein extracts were analysed using mass spectrometry.

Findings: Strains from ribotypes 012 and 027 had the best adhesion when incubated with glucose supplementations (0.2%, 0.5%, and 1%), while RT135 had a poor adherence. The criteria were not met by RT014 and RT133. In the absence of glucose, there was no adhesion for any ribotype, implying that glucose is required and plays a significant role in adhesion.

Main conclusions: These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein.

MeSH terms

  • Clostridioides
  • Clostridioides difficile*
  • Extracellular Matrix
  • Glucose
  • Laminin
  • Ribotyping


  • Laminin
  • laminin 1
  • Glucose