Acridone Derivatives from Atalantia monophyla Inhibited Cancer Cell Proliferation through ERK Pathway
- PMID: 35744993
- PMCID: PMC9228231
- DOI: 10.3390/molecules27123865
Acridone Derivatives from Atalantia monophyla Inhibited Cancer Cell Proliferation through ERK Pathway
Abstract
The present study aimed to investigate the effect of acridone alkaloids on cancer cell lines and elucidate the underlying molecular mechanisms. The ten acridone alkaloids from Atalantia monophyla were screened for cytotoxicity against LNCaP cell lines by a WST-8 assay. Then, the most potential acridone, buxifoliadine E, was evaluated on four types of cancer cells, namely prostate cancer (LNCaP), neuroblastoma (SH SY5Y), hepatoblastoma (HepG2), and colorectal cancer (HT29). The results showed that buxifoliadine E was able to significantly inhibit the proliferation of all four types of cancer cells, having the most potent cytotoxicity against the HepG2 cell line. Western blotting analysis was performed to assess the expression of signaling proteins in the cancer cells. In HepG2 cells, buxifoliadine E induced changes in the levels of Bid as well as cleaved caspase-3 and Bax through MAPKs, including Erk and p38. Moreover, the binding interaction between buxifoliadine E and Erk was investigated by using the Autodock 4.2.6 and Discovery Studio programs. The result showed that buxifoliadine E bound at the ATP-binding site, located at the interface between the N- and C-terminal lobes of Erk2. The results of this study indicate that buxifoliadine E suppressed cancer cell proliferation by inhibiting the Erk pathway.
Keywords: Akt; Erk; apoptosis; buxifoliadine E; cancer cells; p38.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
Similar articles
-
A novel acridone akaloid from Atalantia buxifolia.Nat Prod Res. 2013;27(21):1956-9. doi: 10.1080/14786419.2013.796470. Epub 2013 May 31. Nat Prod Res. 2013. PMID: 23721230
-
Potential anti-allergic acridone alkaloids from the roots of Atalantia monophylla.Phytochemistry. 2008 Oct;69(14):2616-20. doi: 10.1016/j.phytochem.2008.08.007. Epub 2008 Sep 23. Phytochemistry. 2008. PMID: 18817938
-
Cytotoxicity of a naturally occurring furoquinoline alkaloid and four acridone alkaloids towards multi-factorial drug-resistant cancer cells.Phytomedicine. 2015 Sep 15;22(10):946-51. doi: 10.1016/j.phymed.2015.07.002. Epub 2015 Jul 17. Phytomedicine. 2015. PMID: 26321744
-
Acridone Alkaloids.Alkaloids Chem Biol. 2017;78:1-108. doi: 10.1016/bs.alkal.2017.06.001. Epub 2017 Jul 12. Alkaloids Chem Biol. 2017. PMID: 28838426 Review.
-
[Search of novel bioactive natural products from plant sources--novel structures and biological activities--].Yakugaku Zasshi. 2009 Oct;129(10):1155-75. doi: 10.1248/yakushi.129.1155. Yakugaku Zasshi. 2009. PMID: 19797871 Review. Japanese.
Cited by
-
SALL4 correlates with proliferation, metastasis, and poor prognosis in prostate cancer by affecting MAPK pathway.Cancer Med. 2023 Jun;12(12):13471-13485. doi: 10.1002/cam4.5998. Epub 2023 Apr 29. Cancer Med. 2023. PMID: 37119046 Free PMC article.
References
-
- GBD 2015 Risk Factors Collaborators Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990–2015: A systematic analysis for the Global Burden of Disease Study 2015. Lancet. 2016;388:1659–1724. doi: 10.1016/S0140-6736(16)31679-8. - DOI - PMC - PubMed
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous
