Contrary to the long-standing opinion of boronic acids being typically reactive with 1,2- and 1,3-diols and hence not suitable for quantitative sensing of DNA containing only a mono-ol unit, this proof-of-concept study has successfully shown the feasibility to quantitatively detect DNA in the concentration range of 5 to 50 nM plausibly through boronic acid-mediated bridging of two DNA double helices via the 3' hydroxy groups, which opens up new avenues in the realm of oligonucleotide biochemistry.