One-Step Thermophoretic AND Gate Operation on Extracellular Vesicles Improves Diagnosis of Prostate Cancer

Jinqi Deng; Shuai Zhao; Junhong Li; Yangchang Cheng; Chao Liu; Zheng Liu; Lele Li; Fei Tian; Bo Dai; Jiashu Sun

doi:10.1002/anie.202207037

One-Step Thermophoretic AND Gate Operation on Extracellular Vesicles Improves Diagnosis of Prostate Cancer

Angew Chem Int Ed Engl. 2022 Aug 15;61(33):e202207037. doi: 10.1002/anie.202207037. Epub 2022 Jul 11.

Authors

Jinqi Deng^{1

2}, Shuai Zhao^{1

2}, Junhong Li^{3

4}, Yangchang Cheng^{1

2}, Chao Liu^{1

2}, Zheng Liu^{3

4}, Lele Li^{1

2}, Fei Tian^{1

2}, Bo Dai^{3

4}, Jiashu Sun^{1

2}

Affiliations

¹ Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology, Beijing, 100190, China.
² School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
⁴ Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.

PMID: 35749531
DOI: 10.1002/anie.202207037

Abstract

Circulating extracellular vesicles (EVs) have emerged as a valuable source of cancer biomarkers. However, the high degree of EV heterogeneity and the complexity of clinical samples pose a challenge in the sensitive identification of tumor-derived EVs. Here we introduce a one-step thermophoretic AND gate operation (Tango) assay that integrates polyethylene glycol (PEG)-enhanced thermophoretic accumulation of EVs and simultaneous AND gate operation on EV membranes by dual-aptamers recognition. By using the Tango assay to detect tumor-derived EVs with co-presence of EpCAM and PSMA directly from serum in a homogeneous, separation-free format, we can discriminate prostate cancer (PCa) patients from benign prostatic hyperplasia (BPH) patients in the diagnostic gray zone with an accuracy of 91 % in 15 min. Our approach streamlines EV enrichment and AND gate operation on EVs in a single assay, providing a rapid, straightforward, and powerful method for precise and non-invasive diagnosis of cancer.

Keywords: AND Gate; Aptamer; Cancer Diagnosis; Extracellular Vesicles; Thermophoresis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor
Extracellular Vesicles*
Humans
Male
Polyethylene Glycols
Prostatic Neoplasms* / diagnosis

Substances

Biomarkers, Tumor
Polyethylene Glycols