Commercial yerba mate (Ilex paraguariensis) produced in South America: Determination of dithiocarbamate residues by gas chromatography-mass spectrometry

Food Chem. 2022 Nov 15;394:133513. doi: 10.1016/j.foodchem.2022.133513. Epub 2022 Jun 17.


This work presents an easy and quick miniaturized procedure for the analysis of dithiocarbamates fungicides (DTC), by GC-MS, in yerba mate, an important product in South America. The sample amount, the volume of extracting solvent and acid solution, and the time of hot bath shaking were minimized. A clean-up procedure based on PSA, GCB and MgSO4 was studied in order to improve analytical signal and reduce GC-MS system damage. Blank yerba mate samples were spiked with thiram at equivalent CS2 concentrations of 0.1, 0.3, 0.5 and 1 mg kg-1. The validated method at least 2 times faster than the traditional method and was according to the latest European guidelines for pesticide residue analysis. Linearity, limits of detection and quantification, matrix effects, trueness and precision were assessed. Sixty-five commercialized samples from southern Brazil and Argentina were analyzed; 14% of samples presented CS2 concentration greater than LOQ (0.1 mg kg-1).

Keywords: Carbon disulfide; Food safety; Fungicides; Herbs; Survey; Tea.

MeSH terms

  • Argentina
  • Brazil
  • Gas Chromatography-Mass Spectrometry
  • Ilex paraguariensis* / chemistry
  • Pesticide Residues*


  • Pesticide Residues