Effects of two types nitrogen sources on humification processes and phosphorus dynamics during the aerobic composting of spent mushroom substrate

Abstract

Aerobic composting is increasingly regarded as a promising technology for the recycling of spent mushroom substrate (SMS), and an applicable nitrogen source is necessary to improve the process. This study is the first to investigate the effects of protein-like N source (chicken manure, CM) and high-N source (urea, UR) on humification process and P dynamics during SMS composting. The effect of different N sources on microbial succession was also studied. Results showed that CM addition achieved a longer thermophilic phase (16 d vs 9 d), greater germination indices (131.6% vs 106.3%), and higher total phosphorus content (13.1 g/kg vs 6.56 g/kg) in the end products, as compared to UR. The addition of CM showed beneficial effects on humification and stabilization, including decreased weight loss and fluctuations in the level of functional groups. The P produced in the compost was interconverted and leached in the P pool. In this case, the P detected in the compost was in the form of orthophosphate and MgNH₄PO₄⋅6H₂O crystal as inorganic P and orthophosphate monoester as organic P. The most abundant microorganisms at the phylum level mainly include Firmicutes, Actinobacteria, and Proteobacteria, accounting for more than 88% of the total microorganisms. The addition of CM to SMS compost resulted in higher organic matter degradation rates. This work clarified the role of various N sources in SMS composting and presented an appropriate waste management method beneficial to bioresource technology and sustainable development of the edible fungi business.

Keywords: Composting; Microbial community; N source supply; P dynamics; Spent mushroom substrate.