Inhibition of miR-let-7i Induces DC Immature Cells and Improves Skin Graft Tolerance

Dis Markers. 2022 Jun 15;2022:8605621. doi: 10.1155/2022/8605621. eCollection 2022.

Abstract

Dendritic cells (DC) initiate the immune response in the body. They can stimulate T cell activation, proliferation, and differentiation and ultimately participate in the immune response and the immune tolerance response. The purpose of this study was to coculture DCs and T cells and subcutaneously inject DCs transfected with miR-let-7i into rhesus monkey transplantations to verify the role of miR-let-7i in allograft immune tolerance. In vitro studies found that the expression of miR-let-7i was upregulated after inducing the maturation of DCs. The low expression of miR-let-7i inhibited the maturation of DCs, promoted the differentiation of T cells into T helper T cells 2 (Th2), and inhibited T helper T cell 1- (Th1-) driven rejection. In vivo studies also obtained similar results, and subcutaneous injection of DCs transfected with miR-let-7i inhibitor prolonged the survival time of allogeneic skin transplantation. Therefore, we conclude that inhibition of miR-let-7i inhibits DC maturation and improves the tolerance of grafted skin.

MeSH terms

  • Cell Differentiation / immunology
  • Dendritic Cells* / immunology
  • Humans
  • Immune Tolerance
  • MicroRNAs* / antagonists & inhibitors
  • MicroRNAs* / immunology
  • Skin Transplantation*
  • T-Lymphocytes* / immunology
  • Transplantation Immunology

Substances

  • MicroRNAs