Mitochondrial Transfer Regulates Bioenergetics in Healthy and COPD Airway Smooth Muscle

Am J Respir Cell Mol Biol. 2022 Jun 28. doi: 10.1165/rcmb.2022-0041OC. Online ahead of print.

Abstract

Mitochondrial dysfunction has been reported in chronic obstructive pulmonary disease (COPD). Transfer of mitochondria from mesenchymal stem cells to airway smooth muscle cells (ASMCs) can attenuate oxidative stress-induced mitochondrial damage. It is not known whether mitochondrial transfer occurs between structural cells in the lungs nor what role this may have in modulating bioenergetics and cellular function in healthy and COPD airways. Here, we show that ASMCs from both healthy ex-smoker and COPD subjects can exchange mitochondria, a process that happens, at least partly, via extracellular vesicles. Exposure to cigarette smoke induces mitochondrial dysfunction and leads to an increase in the donation of mitochondria by ASMCs, suggesting the latter may be a stress response mechanism. Healthy ex-smoker ASMCs that receive mitochondria show an increase in mitochondrial biogenesis and respiration and a reduction in cell proliferation, irrespective of whether the mitochondria are transferred from healthy ex-smoker or COPD ASMCs. Our data indicate that mitochondrial transfer between structural cells is a homeostatic mechanism for the regulation of bioenergetics and cellular function within the airways and may represent an endogenous mechanism for reversing the functional consequences of mitochondrial dysfunction in diseases such as COPD.

Keywords: COPD; airway smooth muscle cells; bioenergetics; extracellular vesicles.