Processing human skin samples for single-cell assays

STAR Protoc. 2022 Jun 17;3(3):101470. doi: 10.1016/j.xpro.2022.101470. eCollection 2022 Sep 16.


Characterizing resident immune cells in human skin using single-cell assays provides insight into their role in infections, inflammation, and cancer. We describe an optimized protocol to rapidly isolate viable cells from 6-mm skin punch-biopsies. We provide an example in which we coupled single-cell RNA sequencing (scRNA-seq) with single-cell T-cell receptor sequencing (scTCR-seq) of skin and blood cells to study transcriptional profiles and clonotypes of skin resident and peripheral circulating, memory, and effector T cells. This is an improved protocol based on Saluzzo et al. (2021). For complete details on the use and execution of this protocol, please refer to Saluzzo et al. (2021).

Keywords: Cell isolation; Health Sciences; Immunology; RNAseq; Sequencing; Single Cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • T-Lymphocytes*