Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Cortical Stroke with Persistent Sensorimotor Deficits

Margarita Lui; Ayden Gouveia; Diane Lagace; Jing Wang

doi:10.1007/978-1-0716-2409-8_5

Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Cortical Stroke with Persistent Sensorimotor Deficits

Methods Mol Biol. 2022:2515:75-87. doi: 10.1007/978-1-0716-2409-8_5.

Authors

Margarita Lui^{1

2}, Ayden Gouveia³, Diane Lagace^{2

4

5}, Jing Wang^{6

7

8

9}

Affiliations

¹ Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
² Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
³ Max Planck Institute for Metabolism Research, Cologne, Germany.
⁴ University of Ottawa Brain and Mind Research Institute, Ottawa, ON, Canada.
⁵ Canadian Partnership for Stroke Recovery, Ottawa, ON, Canada.
⁶ Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada. jiwang@ohri.ca.
⁷ Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada. jiwang@ohri.ca.
⁸ University of Ottawa Brain and Mind Research Institute, Ottawa, ON, Canada. jiwang@ohri.ca.
⁹ Canadian Partnership for Stroke Recovery, Ottawa, ON, Canada. jiwang@ohri.ca.

PMID: 35776346
DOI: 10.1007/978-1-0716-2409-8_5

Abstract

The endothelin-1 (ET-1) model of stroke involves the stereotactic injection of the vasoconstrictor ET-1 to produce a focal ischemic injury. In rats, this model produces consistent deficits, in contrast to more variable results in mice. In this chapter, we describe a new method to induce a murine focal ischemic cortical stroke by injecting L-NAME, another potent vasoconstrictor , in combination with ET-1 into the sensorimotor cortex. This ET-1 /L-NAME stroke induction protocol produces consistent focal cortical infarcts and sensorimotor functional impairments in C57BL/6 mice.

Keywords: Endothelin-1; Infarct volume; L-NAME; Murine focal ischemia model; Neurological assessment; Stereotactic surgery.

MeSH terms

Animals
Disease Models, Animal
Endothelin-1*
Mice
Mice, Inbred C57BL
NG-Nitroarginine Methyl Ester / pharmacology
Rats
Stroke* / chemically induced
Vasoconstrictor Agents

Substances

Endothelin-1
Vasoconstrictor Agents
NG-Nitroarginine Methyl Ester