Variovorax terrae sp. nov. Isolated from Soil with Potential Antioxidant Activity

J Microbiol Biotechnol. 2022 Jul 28;32(7):855-861. doi: 10.4014/jmb.2205.05018. Epub 2022 Jun 30.

Abstract

A white-pigmented, non-motile, gram-negative, and rod-shaped bacterium, designated CYS-02T, was isolated from soil sampled at Suwon, Gyeonggi-do, Republic of Korea. Cells were strictly aerobic, grew optimally at 20-28ºC and hydrolyzed Tween 40. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain CYS-02T formed a lineage within the family Comamonadaceae and clustered as members of the genus Variovorax. The closest members were Variovorax guangxiensis DSM 27352T (98.6% sequence similarity), Variovorax paradoxus NBRC 15149T (98.5%), and Variovorax gossypii JM-310T (98.3%). The principal respiratory quinone was Q-8 and the major polar lipids contain phosphatidylethanolamine (PE), phosphatidylethanolamine (PG), and diphosphatidylglycerol (DPG). The predominant cellular fatty acids were C16:0, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The DNA GC content was 67.7 mol%. The ANI and dDDH values between strain CYS-02T and the closest members in the genus Variovorax were ≤ 79.0 and 22.4%, respectively, and the AAI and POCP values between CYS-02T and the other related species in the family Comamonadaceae were > 70% and > 50%, respectively. The genome of strain CYS-02T showed a putative terpene biosynthetic cluster responsible for antioxidant activity which was supported by DPPH radical scavenging activity test. Based on genomic, phenotypic and chemotaxonomic analyses, strain CYS-02T was classified into a novel species in the genus Variovorax, for which the name Variovorax terrae sp. nov., has been proposed. The type strain is CYS-02T (= KACC 22656T = NBRC 00115645T).

Keywords: Comamonadaceae; Variovorax terrae; antioxidant; soil.

MeSH terms

  • Antioxidants / analysis
  • Bacterial Typing Techniques
  • Comamonadaceae*
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Phosphatidylethanolamines* / analysis
  • Phospholipids / chemistry
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil
  • Soil Microbiology

Substances

  • Antioxidants
  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylethanolamines
  • Phospholipids
  • RNA, Ribosomal, 16S
  • Soil