Isolation of Some Phenolic Compounds from Plantago subulata L. and Determination of Their Antidiabetic, Anticholinesterase, Antiepileptic and Antioxidant Activity

Chem Biodivers. 2022 Aug;19(8):e202200280. doi: 10.1002/cbdv.202200280. Epub 2022 Jul 7.

Abstract

In the current study, some phenolic compounds, including acteoside, isoacteoside, echinacoside, and arenarioside purified and characterized from Plantago subulata. These compounds were tested for its antioxidant potential, including Fe3+ and Cu2+ reductive ability and Fe2+ chelating effects. The inhibitory effects of isolated phenolic compounds were tested towards human carbonic anhydrase I and II isoenzymes (hCA I and hCA II), butyrylcholinesterase (BChE) acetylcholinesterase (AChE), aldose reductase (AR) and α-glycosidase (α-gly). Ki values were found these compounds in range of 0.24±0.05-1.38±0.34 μM against hCA I, 0.194±0.018-1.03±0.06 μM against hCA II, 0.043±0.01-0.154±0.02 μM against AChE, 3.92±1.08-11.93±4.45 μM against BChE, 0.082±0.0008-1.68±0.42 μM against AR, and 6.93±2.74-17.17±6.70 μM against α-glycosidase. As a result, isolated compounds displayed inhibition effects against studied all metabolic enzymes. They are promising candidates for treating disorders like Alzheimer's disease, diabetes mellitus, glaucoma, leukemia, and epilepsy.

Keywords: Isolation; anticholinesterase; antidiabetic; antioxidant; enzyme inhibition; phenolic compounds.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Anticonvulsants / pharmacology
  • Antioxidants / pharmacology
  • Butyrylcholinesterase / metabolism
  • Carbonic Anhydrase Inhibitors / pharmacology
  • Cholinesterase Inhibitors* / pharmacology
  • Glycoside Hydrolases / metabolism
  • Humans
  • Hypoglycemic Agents / pharmacology
  • Molecular Structure
  • Plantago* / metabolism
  • Structure-Activity Relationship

Substances

  • Anticonvulsants
  • Antioxidants
  • Carbonic Anhydrase Inhibitors
  • Cholinesterase Inhibitors
  • Hypoglycemic Agents
  • Acetylcholinesterase
  • Butyrylcholinesterase
  • Glycoside Hydrolases