Transformation of yeast by a replicating hybrid plasmid

Nature. 1978 Sep 14;275(5676):104-9. doi: 10.1038/275104a0.

Abstract

Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from E. coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.

MeSH terms

  • DNA, Recombinant*
  • Escherichia coli / genetics*
  • Hybridization, Genetic
  • Methods
  • Plasmids*
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*

Substances

  • DNA, Recombinant