Local synthesis of the phosphatidylinositol-3,4-bisphosphate lipid drives focal adhesion turnover

Dev Cell. 2022 Jul 25;57(14):1694-1711.e7. doi: 10.1016/j.devcel.2022.06.011. Epub 2022 Jul 8.


Focal adhesions are multifunctional organelles that couple cell-matrix adhesion to cytoskeletal force transmission and signaling and to steer cell migration and collective cell behavior. Whereas proteomic changes at focal adhesions are well understood, little is known about signaling lipids in focal adhesion dynamics. Through the characterization of cells from mice with a kinase-inactivating point mutation in the class II PI3K-C2β, we find that generation of the phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4)P2) membrane lipid promotes focal adhesion disassembly in response to changing environmental conditions. We show that reduced growth factor signaling sensed by protein kinase N, an mTORC2 target and effector of RhoA, synergizes with the adhesion disassembly factor DEPDC1B to induce local synthesis of PtdIns(3,4)P2 by PI3K-C2β. PtdIns(3,4)P2 then promotes turnover of RhoA-dependent stress fibers by recruiting the PtdIns(3,4)P2-dependent RhoA-GTPase-activating protein ARAP3. Our findings uncover a pathway by which cessation of growth factor signaling facilitates cell-matrix adhesion disassembly via a phosphoinositide lipid switch.

Keywords: ARAP3; DEPDC1B; PKN2; RhoA signaling; cell migration; class II PI3K; focal adhesion; lipid switch; phosphatidylinositol-3,4-bisphosphate; phosphoinositide.

MeSH terms

  • Animals
  • Cell Adhesion
  • Focal Adhesions* / metabolism
  • Mice
  • Phosphatidylinositol 3-Kinases / metabolism
  • Phosphatidylinositol Phosphates / metabolism
  • Phosphatidylinositols* / metabolism
  • Proteomics


  • Phosphatidylinositol Phosphates
  • Phosphatidylinositols