The coordination of V-ATPase and ATG16L1 is part of a common mechanism of non-canonical autophagy

Autophagy. 2022 Oct;18(10):2267-2269. doi: 10.1080/15548627.2022.2100678. Epub 2022 Jul 19.


The conjugation of Atg8-family proteins with phospholipids on the double-membrane phagophore is one of the hallmarks of macroautopahgy/autophagy. However, in the past decades, Atg8-family proteins have also been identified on single-membrane structures, including the phagosome, endosome and lysosome. While the physiological importance of the non-canonical Atg8-family protein conjugation has been demonstrated, the mechanism of this process and the underlying regulation are still not very clear. In a recent paper, Hooper et al. found that during LC3-associated phagocytosis, reactive oxygen species are required for V-ATPase assembly, which is essential for the subsequent LC3 conjugation to the phagosome. Enhanced V-ATPase assembly and the direct engagement of ATG16L1 are also observed in a wide range of non-canonical Atg8-family protein conjugation processes, defining the V-ATPase and ATG16L1 as taking part in a common mechanism.

Keywords: ATG16L1; Atg8 conjugation; LAP; ROS; V-ATPase; non-canonical autophagy.

Publication types

  • Editorial
  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenosine Triphosphatases*
  • Autophagy*
  • Autophagy-Related Protein 8 Family / metabolism
  • Autophagy-Related Proteins / metabolism
  • Microtubule-Associated Proteins / metabolism
  • Reactive Oxygen Species


  • Autophagy-Related Protein 8 Family
  • Autophagy-Related Proteins
  • Microtubule-Associated Proteins
  • Reactive Oxygen Species
  • Adenosine Triphosphatases