pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long

Nucleic Acids Res. 1978 Aug;5(8):2721-8. doi: 10.1093/nar/5.8.2721.

Abstract

I have derived a complete restriction map of pBR322 from the total nucleotide sequence of the plasmid. Most of the restriction sites also have been demonstrated empirically. The exact sizes of all restriction fragments and the relative positions of the cuts are presented. These fragments can serve as accurate DNA size markers from small pieces up to the 4362 base pair length of pBR322. Inserts cloned in this vector may be characterized easily using this data.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Restriction Enzymes / metabolism
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics
  • Molecular Weight
  • Plasmids*
  • R Factors

Substances

  • DNA, Bacterial
  • DNA Restriction Enzymes