Comprehensive metabolome analysis of intracellular metabolites in cultured cells

STAR Protoc. 2022 Sep 16;3(3):101531. doi: 10.1016/j.xpro.2022.101531. Epub 2022 Jul 11.


Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types.

Keywords: Cell Biology; Cell culture; Mass Spectrometry; Metabolism; Metabolomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cells, Cultured
  • Mass Spectrometry / methods
  • Metabolome*
  • Metabolomics* / methods